Table 1.
Comparison of HRM, DHPLC, Sequencing and Real-Time Allele-Specific PCR for the Detection of the BRAF V600E Mutation in the Learning Set of Archival Colorectal Cancer Samples
| Sample ID | HRM | DHPLC | Sequencing | Real-time allele-specific PCR |
|---|---|---|---|---|
| P2 | mt | mt | mt | mt |
| P13 | mt | mt | mt | mt |
| P15 | mt | mt | mt | mt |
| P16 | mt | mt | mt | mt |
| P17 | mt | mt | mt | mt |
| P19 | mt | wt | mt | mt |
| P20 | wt | wt | wt | wt |
| P22 | mt | mt | mt | mt |
| P23 | mt | mt | mt | mt |
| P24 | mt | mt | mt | mt |
| P26 | wt | wt | wt | wt |
| P30 | mt | mt | mt | mt |
| P45 | mt | mt | mt | mt |
mt = BRAF V600E mutant; wt = wild-type.