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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Chem Res Toxicol. 2008 Nov;21(11):2188–2194. doi: 10.1021/tx800265g

Figure 1. Reduction of Cr(VI) and formation of Cr-DNA adducts in reactions containing 2 and 5 mM GSH.

Figure 1

Reaction mixtures were incubated for 1 hr at 37°C followed by immediate absorbance measurements or purification of DNA by Bio-Gel P-30 chromatography. Cr-DNA adducts were measured by GF-AAS. Where not seen, error bars were smaller than symbols. (A) Reduced amounts of Cr(VI) after incubation with 2 or 5 mM GSH in 25 mM MOPS or phosphate (Pi) buffer. Data are means from 3 independent determinations. (B) Formation of Cr-DNA adducts in reactions containing 2 or 5 mM GSH, 0–200 µM Cr(VI), 2 µg pSP189 DNA and 25 mM MOPS or phosphate (Pi) buffer. Means±SD from four independent determinations. (C) Yield of Cr-DNA adducts as a function of reduced Cr(VI) in reactions containing 2 or 5 mM GSH.