Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Chem Res Toxicol. 2008 Nov;21(11):2188–2194. doi: 10.1021/tx800265g

Figure 5. Formation of GSH-Cr-DNA crosslinks during Cr(VI) reduction by GSH.

Figure 5

Cr(VI) reduction was performed in 25 mM MOPS buffer, pH 7.0. Total Cr-DNA adducts were measured by GF-AAS and the amount of DNA-crosslinked GSH was determined either by the inclusion of 35S-GSH or by a phosphate dissociation-HPLC procedure. (A) Levels of GSH-DNA crosslinks generated in Cr(VI) reactions containing 2 or 5 mM GSH. HPLC – GSH crosslinks were quantified by HPLC, 35S – GSH crosslinks were measured using radioactive GSH. Data are means±SD from 4–8 independent determinations. (B) Relative yield of GSH-Cr-DNA crosslinks in reactions containing 2 and 5 mM GSH. Data are GSH-DNA crosslinks as the percentage of total Cr-DNA adducts.