Skip to main content
. Author manuscript; available in PMC: 2009 Nov 4.
Published in final edited form as: Biochemistry. 2008 Oct 9;47(44):11682–11692. doi: 10.1021/bi801451f

Figure 3. Transcriptional down regulation of AdipoR1 and AdipoR2 attenuates adiponectin-mediated ERK1/2 activation.

Figure 3

A) Quantification of AdipoR1 and AdipoR2 mRNA level by real time PCR. HEK293 cells were transfected with scrambled control siRNA (CNR) or siRNA targeting AdipoR1 (ADR-1), AdipoR2 (ADR-2) or both (ADR-1+2) as described and the efficiency of transcriptional down regulation was assessed by quantitative real time PCR. The abundance of AdipoR1 and AdipoR2 mRNA in each sample was normalized to the mRNA expression levels of the housekeeping gene, Cyclophilin A. B) Effect of adiponectin receptor downregulation on adiponectin-stimulated ERK1/2 activation. Serum deprived cells were stimulated for 5 min with vehicle or full-length adiponectin (8 µg/ml) and ERK1/2 activity was determined by immunoblotting with anti-phospho-ERK1/2. ERK1/2 phosphorylation is expressed as the fold increase above the basal level in unstimulated, CNR-transfected cells. A representative phospho-ERK1/2 immunoblot is shown above a bar graph representing the Mean ± SEM from six independent experiments. ** less than CNR treated, p<0.005.