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. 2000 Jan 4;97(1):309–314. doi: 10.1073/pnas.97.1.309

Figure 1.

Figure 1

Molecular characterization of the t(1;22)(q22;11) breakpoint in follicular lymphoma patient Co. (A) Southern blot analysis of IGLC rearrangements in B593 compared with original patient material (Co) using the pJλ2 probe, which detects a polymorphic EcoRI fragment encompassing the IGLC2 and IGLC3 genes (26). Short arrows indicate rearranged bands. Co is heterozygous for the 18- and 8-kb alleles; a faint 8-kb band visible in Co (horizontal arrow) but not in B593 corresponds to detection of the 8-kb germline band in normal cells in the biopsy material. In the tumor cells, the 18-kb allele is retained whereas rearrangement of the 8-kb fragment after translocation generates an aberrant 2.2-kb band. (B) Genomic sequence surrounding the der(1) breakpoint of the t(1;22)(q22;q11). The translocation juxtaposes germline chromosome 1 sequence to a somatically hypermutated V gene. A vertical arrow indicates the breakpoint site, and the Chi-like sequences are underlined. (C) Southern blot analysis of the der(1) breakpoint in B593. The p607/21–68 covering the IGLC2/3 genes and the 1q22 breakpoint-specific probe EB1 detect identical rearranged fragments (arrows).