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. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: Cell Cycle. 2008 Oct 25;7(20):3262–3272. doi: 10.4161/cc.7.20.6852

Figure 5.

Figure 5

The cdc14(1–374) allele is hypomorphic. (A) Cells carrying Cdc14-3HA (A1411) or Cdc14(1–374)-3HA fusions (A19448) were grown as described in Figure 1B. The percentage of cells in metaphase (open circles) and anaphase (closed squares) was determined at the indicated time points. (B) FEAR network signaling is essential in cdc14(1–374)-3HA mutants. Cells of the indicated genotype were grown in YEP media containing 2% raffinose-2% galactose, serially diluted 10-fold, and spotted on solid YEP media containing 2% raffinose-2% galactose (Raf-Gal) or 2% glucose (Glc). The plates were incubated at 30°C for 2 days. The following strains were used (from top to bottom): wild-type cells carrying a Cdc14-3HA fusion (A1411), cdc14(1–374)-3HA (A19448) cells, pGAL-URL-3HA-SPO12 cells (A12737), and pGAL-URL-3HA-SPO12 cdc14(1–374)-3HA (A20798) cells. (C) cdc14(1–374)-3HA is not synthetically lethal with cdc55Δ. 10-fold serial dilution of wild-type (A2587), cdc14(1–374)-3HA (A19448), cdc55Δ (A15396), and cdc14(1–374)-3HA cdc55Δ (A20810) cells on solid YEPD media. Cells were grown at 30°C for 2 days. (D) cdc14(1–374)-3HA enhances the temperature sensitivity of cdc15-2 and dbf2-2 mutants. Cells of the indicated genotype were spotted on solid YEPD media and grown for 2 days at room temperature (RT), 33°C, or 37°C. The following strains were used (from top to bottom): A1411, A19448, A1674, A19765, A2505, A20873. A1411, A1674 and A2505 carry a Cdc14-3HA fusion.