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. Author manuscript; available in PMC: 2010 Mar 18.
Published in final edited form as: FEBS Lett. 2009 Feb 21;583(6):1023–1028. doi: 10.1016/j.febslet.2009.02.027

Fig. 2.

Fig. 2

Real-time PCR validation of genes regulated by Tie-1 expression. Gene expression, normalized to GAPDH level, was determined by real-time PCRs and presented as mean±SD. Expression level of the gene of interest when HUVECs were treated with the control siRNA was arbitrarily set to one and used for normalization. *Tie-1 siRNA#3 appeared to partially knock down Tie-2 expression (see text for explanations). RNAs from three siRNA transfections were tested (n=3). P values were determined by t-tests.