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. 2009 Feb 5;28(6):652–662. doi: 10.1038/emboj.2009.15

Figure 1.

Figure 1

The Mre11 and Nbs1 proteins are required for robust ATR signaling in response to mutant Ad infection. (A) Infections were performed in a transformed cell line deficient for Mre11 (A-TLD1) that was transduced with an empty retrovirus vector (vector) or a vector expressing the wild-type Mre11 cDNA (Mre11). Cells were uninfected (Mock) or infected with wild-type Ad5 (Ad5) and the E4-deletion mutant (dl1004). Immunoblotting of lysates prepared at 30 hpi used antibodies for total protein (Chk1, RPA32, Mre11, Rad50 and Nbs1) or phospho-specific sites (Chk1-S345). Ku86 served as a loading control. (B) Infections were performed in the transformed NBS cell line (NBS-ILB1) transduced with an empty retrovirus vector (vector) or a vector expressing the wild-type Nbs1 cDNA.