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. 2009 Feb 5;28(6):652–662. doi: 10.1038/emboj.2009.15

Figure 2.

Figure 2

Phosphorylation of ATR substrates is reduced in the presence of Ad5-E4orf3 and is independent of ATM. (A) Genotypes of mutant viruses used for infection (Bridge and Ketner, 1990). (B) HeLa cells were mock infected or infected with indicated viruses. Cells were harvested at 6, 12, 18 and 24 hpi and prepared for analysis by immunoblotting using antibodies to phospho-specific residues (Chk1-S345, RPA32-S4,8 and ATM-S1981). Below are lysates at 24 hpi probed with antibodies against total protein (Chk1, RPA32 and Ku86) to serve as loading controls. (C) Immunoblots of infections in control cells (48BR) or Seckel cells with mutant ATR (GM18366). (D) Immunoblots of infections in A-T cells with mutant ATM (AT221JE-T) or a complemented line (A-T+ATM). DBP was a marker of virus infection and Ku86 served as a loading control.