Skip to main content
. 2009 Feb 5;28(6):652–662. doi: 10.1038/emboj.2009.15

Figure 6.

Figure 6

E4orf3 abrogates MRN function by immobilizing the MRN complex. (A) HeLa cells were transfected with Nbs1–YFP or Mre11–YFP alone or together with Ad5-E4orf3 plasmid vector. After 24 h, cells were infected with dl1004 or dl1017. Cells were fixed 18 hpi, and immunofluorescence was performed with a Rad50 antibody. Images shown are merged with DAPI staining. (B–D) FRAP analysis of Nbs1 and Mre11 in cells expressing E4orf3 by infection or transfection. The unbleached portion of the cell served to normalize the overall fluorescence decay during the repeated image collection. Arrows above indicate the time of bleaching. (B) Stable U2OS cells expressing Nbs1–2GFP were either untreated, transfected with E4orf3, or treated with 10 Gy gamma-irradiation. (C) HeLa cells were transfected with Nbs1–YFP and then mock treated or infected with dl1004 and dl1017. Cells were analyzed by FRAP at 18 hpi. (D) HeLa cells transfected with Mre11–YFP together with either empty vector or vectors expressing Ad5-E4orf3 and E4orf3-I104R were analyzed by FRAP.