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. 2009 Feb 5;28(6):632–640. doi: 10.1038/emboj.2009.9

Figure 5.

Figure 5

Mitochondrial-generated ROS induce the death of tdp1Δ cells. (A) Vitamin B6 (or PLP), vitamin C (vit C) and N-acetyl-L-cysteine (NAC) protect the quiescent tdp1Δ cells. The strains are incubated for 24 h in G0 and then treated for 24 h with pyridoxal 5′-phosphate (PLP), vitamin C or N-acetyl-L-cysteine before being plated and incubated for 3 days. (B) PLP counteracts the deleterious effects of the superoxide generator MSB. PLP was introduced before MSB in the culture. The procedure is the same as above (for (A, B), more than 200 colonies are counted for each condition and the experiments have been repeated three times independently). (C) The low respiration rate observed in the tsf1Δ mutant protects the quiescent tdp1Δ cells. Plating efficiencies after 2, 4 and 8 days in G0 of quiescent wild-type, tdp1Δ, tsf1Δ and tsf1Δ tdp1Δ strains (more than 500 colonies are counted for each strain, n=3 independent experiments). (D) FACS analyses of the 8-day-starved tsf1Δ and tsf1Δ tdp1Δ mutant strains showing the lack of propidium iodide-negative cells. The percentage (%) of propidium iodide-negative cells is indicated for each strain.