Ovaries from PND4 Fischer 344 neonatal rats were cultured with vehicle control or the intermediate metabolite DMBA-3,4-diol (12.5 nM - 1 μM) for 15 days. Following incubation, ovaries were collected, and processed for histological evaluation as described in methods. Healthy primordial (A) and primary (B) follicles were classified and counted. Values are mean ± SE total follicles counted per ovary, n=5; * = different from control in each follicle type, P < 0.05.