Ovaries from PND4 Fischer 344 rats were cultured with (A) vehicle control or media containing DMBA (1 μM) +/− CHO (2 mM) for 4 days; or (B) vehicle control or media containing DMBA-3,4-diol (75 nM) +/− CHO (2 mM) for 4 days. Following incubation, ovaries were collected, processed for histological evaluation and healthy follicles were counted as described in methods. Values are mean ± SE total follicles counted/ovary, n=5; * = different from control in each follicle type, P < 0.05.