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. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2004 Aug;45(8):2569–2576. doi: 10.1167/iovs.03-1323

FIGURE 5.

FIGURE 5

Effects of blocking EGFR-mediated pathways on the cleavage of PARP in P. aeruginosa–infected HUCL cells and primary HCECs. Cells were preincubated with 250 nM AG1478, 10 μM LY294002, or 5 μM U0126 for 30 minutes and challenged with P. aeruginosa at a cell-to-bacterium ratio of 1:25 in the presence of the inhibitors for 5 hours. Cells were lysed and 15 μg cell lysates were immunoblotted with anti-PARP antibody. cPARP represents the cleaved form. To normalize protein loading, anti-ERK2 was applied as probe to the same membrane.