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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Brain Behav Immun. 2009 Jan 8;23(4):455–463. doi: 10.1016/j.bbi.2009.01.001

Figure 7. siRNA inhibition of STAT5 protein expression does not alter DEX-induced MMTV-luciferase activity in the absence of mIFN-alpha treatment.

Figure 7

Panel A: HT22 cells were grown in 12-well culture plates until 80% confluent. Cells were then transiently transfected for 24 hours with 100 nM of siRNA directed against STAT5, or were transfected with a non-target siRNA, followed by transfection with the MMTV-luciferase reporter construct. Following transfection, cells were then treated with vehicle for 24 hours, mIFN-alpha (1000 U/ml) for 24 hours, vehicle for 22 hours followed by vehicle plus DEX (50 nM) for 2 hours, and mIFN-alpha for 22 hours followed by mIFN-alpha plus DEX for 2 hours. Values shown represent the mean (± SEM) of observations obtained from three independent experiments. * p<0.05 vs. cells treated with DEX alone; ** p<0.05 vs. cells treated with mIFN-alpha plus DEX.