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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Brain Behav Immun. 2009 Jan 8;23(4):455–463. doi: 10.1016/j.bbi.2009.01.001

Figure 8. STAT5 and GR engage in protein-protein interactions after treatment with IFN-alpha and dexamethasone in HT22 cells.

Figure 8

Images are representative of three separate experiments. Nuclear extracts were generated from cells treated with vehicle, mIFN-alpha (1000 U/ml), or mIFN-alpha plus DEX (50 nM) for 30 minutes and immunoprecipitated using protein A-or protein-G-agarose beads with an anti-GR antibody. Proteins were then resolved using 10% Tris-HCl gels, electrophoretically transferred to nitrocellulose membranes, and blotted with anti-p-STAT5 or anti-GR antibodies.