Fig 3.

Induction of the murine homologue of human β-defensin-2, named murine β-defensin (MBD)-3, in bone after intraosseous contamination with Staph. aureus. Cross-sections of murine shinbones, treated with intraosseous phosphate-buffered saline (PBS) injections showed nearly no immunoreactivity (arrows) to MBD-3 antibody in endostal osteoblasts (C). Application of Staph. aureus into the medullary cavity of murine shinbones resulted in an increased expression of MBD-3 in the pericellular matrix of endostal osteoblasts (A and B). Negative controls were carried out by absorption of the primary antibody by recombinant protein (D).