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. 2009 Apr 16;4(4):e5152. doi: 10.1371/journal.pone.0005152

Figure 2. The effect of species divergence time on divergence-based ascertainment bias.

Figure 2

Coalescent simulation as in Figure 1 were performed, however a species divergence time was varied between 5 and 100 4Ne generations was used (see Figure 1 caption for simulation details). Shown are values of Tajima's D from the upper (blue) and lower (orange) 1% regions. A dotted line at D = 0 is shown for reference to the neutral expectation. The strength of a divergence-based ascertainment bias decreases as a function of species divergence time, but does very slowly such that an appreciable effect remains at species divergence times of 100.