Fig. 1.

Particle size distribution and electrophoretic mobility of DMPC-treated LDL. A: The particle size of LDL in PBS was determined by dynamic light scattering. The LDL was incubated with DMPC (1 mM) at 25°C for 5 min. The particle size distribution was expressed as the intensity of light scattering as a function of particle size. B: Agarose gel electrophoresis of DMPC-treated LDL. Increasing amounts of DMPC vesicles (0.5 to 2 mM) in PBS were incubated with LDL (275 μg/ml protein) at 37°C for 2 h. Samples were subjected to separation with the Helena TITAN™ lipoprotein electrophoresis system and stained with Fat Red 7B.