Fig. 2.

Triglyceride biosynthesis in peritoneal macrophages from PON2-deficient versus control mice. MPMs were harvested from 5-month-old control (C57BL/6) mice and from age-matched PON2-deficient mice. A: MPM (3 × 10⁶) from both groups were incubated for 3 h at 37°C with 6 μCi/ml [³H]oleate bound to fatty-acid-free BSA (2%). The cells were washed and further incubated for up to 4 h at 37°C in DMEM + 0.2% BSA. The level of cellular radiolabeled triglyceride content was determined in the lipid extract after separation of the lipids by TLC at 0 time and after 0.5, 1, and 4 h of incubation. B: MPMs (3 × 10⁶) from both groups were incubated for up to 6 h at 37°C with 6 μCi/ml [³H]oleate bound to fatty-acid-free BSA (2%). After 0.5, 1, 3, and 6 h of incubation, the cells were washed and their lipids were extracted and separated by TLC. The radioactivity in the triglyceride spots was determined by liquid scintillation counting. C, D: MPMs were harvested from PON2-deficient mice and from control mice at the ages of 2, 5, and 9 months. C: The cellular triglyceride content was determined in the TLC spots. D: The extent of triglyceride biosynthesis after 3 h of incubation was determined as described in B. Results are given as mean ± SD of three different experiments. *P < 0.01 versus control MPM.