TABLE 2.
Effects of diosgenin on the lipid composition of gallbladder bile
| Genotype | Treatment | Chol | PL | BS | Chol | PL | BS |
|---|---|---|---|---|---|---|---|
| μmol/ml | % molar | ||||||
| WT | Control | 5.1 (0.4)a | 29 (2)a | 138 (6)a,b | 3.0 (0.3)a | 17 (0.8)a | 80 (0.9)a |
| WT | Diosgenin | 18 (1.3)b | 27 (3)a | 111 (12)a | 12 (0.5)b | 17 (0.8)a | 71 (1)b |
| L1KO | Control | 7.2 (0.2)a | 35 (2)a | 149 (7)b | 3.8 (0.2)a | 18 (0.5)a | 78 (0.5)a |
| L1KO | Diosgenin | 26 (2.5)c | 33 (1)a | 124 (8)a,b | 14 (1.9)b | 18 (0.7)a | 68 (2)b |
After being fed for 14 d the diet supplemented without (control) or with diosgenin (1% w/w), mice were fasted for 4 h and bile was collected from the gallbladder for the determination of biliary concentrations of cholesterol (Chol), phospholipids (PL), and bile salts (BS). Each column represents mean (± SEM) of six samples. Statistically significant differences for each column of values are indicated by different superscript letters (P < 0.05).