TABLE 2.
Apparent kinetic studies of LPGAT activity of ALCAT1
| [14C]Acyl-CoA | Km | Vmax | Vmax/Km |
|---|---|---|---|
| μM | nmol/min/mg protein | ||
| Oleoyl-CoA (18:1) | 54 | 5,514 | 102.11 |
| Linoleoyl-CoA (18:2) | 36 | 1,358 | 37.72 |
| Stearoyl-CoA (18:0) | 70 | 3,530 | 50.43 |
| Palmitoyl-CoA (16:0) | 34 | 1,673 | 49.21 |
LPGAT, lysophosphatidylglycerol acyltransferase. Kinetic data shown in Fig. 3B were used to calculate apparent Km and Vmax.