Fig. 1.

Nuclear export of cytidine triphosphate:phosphocholine cytidylyltransferase-green fluorescent protein (CCT-GFP) in live cells. CHO58 cells transiently expressing CCT-GFP were mounted on a 40°C heated microscope stage. After equilibration for 15–20 min, cells received 60 μM farnesol (FOH), and images were captured at 30 s intervals (150 ms exposures) for 30 min as described in Materials and Methods. The Quicktime movie from which these individual images were extracted is shown in supplementary Fig. I.