Table 1.

HSP and actin gene primer sets for L. elliptica and N. concinna

Organism	Primer set	Gene	Primer sequence		RSq	PCR efficiency (%)
Nacella concinna	1F2 and 1Rev2	HSP70A	Nco1F2	ATTCGATGACGAGACGGTTCA	0.968	134.90
			Nco1Rev2	AACGTCTTCAATTCGCTTTTGTA
	3F and 3Rev	HSP70B	Nco3F	AGTTCACCGACGACACAGTAC	0.945	103.70
			Nco3Rev	TATTTTAGTCTCTGATTTGTACTC
	7F and 7Rev2	GRP78	Nco7F	CTTGGGATGATAAATCTGTCCA	0.996	86.40
			Nco7Rev2	CTTTGTCAGAACCTTGTACATTA
	9F and 9Rev	HSC70	Nco9F	AATTTGACGATGGACACGTTCAA	0.988	87.00
			Nco9Rev	GGTCTTTTGTTCACCCTTGTAG
	ActinF and ActinR4	Actin	NcoActinF	GAGAAATCGTCCGAGACATCAA	0.983	95.00
			NcoActinRev4	CAGCAGATTCCATACCCAAGAA
Laternula elliptica	2F and 2Rev	HSP70A	Lel2F	CTGTCTTGAGCGATGGTGGC	0.998	116.80
			Lel2Rev	TTTGTTACGGTCTTTCCTAAGTA
	3F3 and 3Rev	HSC70	Lel3F3	CAATGACAACACTCGCCCCA	0.996	84.30
			Lel3Rev	TGTTGACAGTCTTTCCGAGGTA
	4F and 4Rev	HSP70B	Lel4F	AAGCTTGTCAACCACGGCGG	0.975	107.20
			Lel4Rev	CCTTGACCCTTTGGCCAAGG
	5F and 5Rev	GRP78	Lel5F	GGTCAAGAACAAGAACAACAAAC	1.000	94.90
			Lel5Rev	TGACGATTTTCTCTCCCAGGAA
	Actin F and ActinRev	Actin	LelActinF	CGACGGTCAGGTCATCACCA	0.999	95.90
			LelActinRev	GACAGGACAGTGTTGGCGTA

RSq and PCR efficiency values are included, as calculated using the Stratagene MxPro–MX3000P v. 3.00 Build 311 Schema 74 software.