Fig. 5.

LiCl affects glioma cell movement in brain slices and induces pronounced changes in glioma cell shape. (A) U87 spheroid invading collagen I matrix. A U87 spheroid was grown for 48 h and then exposed to 20 mM NaCl or LiCl for 24 h and stained with alexafluor-568 phalloidin. The image shows a quadrant of the spheroid (Bar = 100 μm). Inset shows a detailed image of a migrating cell (Bar = 10 μm). (B) X12 glioma spheroid migrating on a brain slice treated with 20 mM NaCl or with LiCl for 24 h. Tumor cells are visualized by immunohistochemical anti-vimentin (green) staining. Cell nuclei stained with Hoechst 33528 are shown in red.