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. 2009 Apr 16;4(4):e5205. doi: 10.1371/journal.pone.0005205

Figure 4. Marker protein levels reflect gene expression in quiescent and activated satellite cells.

Figure 4

Total RNA was obtained from quiescent (T0) and activated (T48) satellite cells and transcript-specific primers used to semi-quantitatively amplify the mRNA of markers. Amplification of Gapdh transcript was used as control of the RNA content. Pax7, MyoD and myogenin transcripts levels were included as control of the activation progression. While caveolin-1 and CTR transcripts appeared to be more abundant in satellite cells at T0, mRNA for integrin α7, Emd and Lmna were unaltered. Jagged-1 and MyoD transcript were increased in activated satellite cells, reflecting the protein levels. Myogenin transcript, as expected, was absent in quiescent satellite cells and appeared after 48 hrs in culture. NTC is no-template control.