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EDL myofibres were cultured in suspension for 72 hrs, by which time many begin to express myogenin as they commit to myogenic differentiation (arrows). Myogenin-containing satellite cells (green – arrowed) tended to have lower levels of caveolin-1 immunosignal (red - arrow) in their plasma membranes than uncommitted cells. Nuclei were revealed by counterstaining with DAPI (blue). Scale bar represents 20 µm.
