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. Author manuscript; available in PMC: 2009 Apr 7.
Published in final edited form as: Nature. 2008 Apr 17;452(7189):840–845. doi: 10.1038/nature06847

Figure 1. Promoter-ORF network rewiring.

Figure 1

a, Example of construct (csgD-CRP), with two ribosome binding sites (RBS). b, Network diagram of the major transcription factor and σ-factor genes used. Green, red and blue arrows denote direct activating, repressing and dual interactions, respectively, from RegulonDB6,7. σ-factors, master regulators and lower-tier regulators are in purple, yellow and beige. Black numbers denote the total number of direct downstream ORF-gene interactions per node. The housekeeping σ-factor RpoD can activate all other nodes. Dotted arrows illustrate two rewired constructs (fliA-flhD and csgD-CRP; e.g. CRP, RpoS, OmpR and CsgD all regulate csgD, thus connecting 4 nodes to CRP in csgD-CRP).