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. 2008 Oct 4;18(1):118–127. doi: 10.1093/hmg/ddn322

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Allele-specific sequencing results for murine Blcap. (A) The two top-most rows of sequencing traces labelled gDNA (genomic DNA) show the B6 (T) and cast (A) genotypes at the site of the T/A SNP between the two strains (Fig. 1B). The other rows contain the traces obtained from amplifying and sequencing Blcap_v1a using adult (ad) and newborn (nb) brain (Br) cDNA samples of B6 × cast (B×C) and cast × B6 (C×B) inter-subspecific hybrids (denoted in dam × sire order). In both adult and newborn brain and for both directions of the cross, the dominant peak in the trace at the site of the SNP corresponds to the maternal allele, indicating Blcap_v1a expression from preferentially the maternal allele. (B) A G/C SNP between B6 and cast (top gDNA traces and Fig. 1B) was used to determine the allelic origin of Blcap_v1a expression in 9.5 dpc embryo (Em). The maternal allele dominated the sequence traces for the B×C and C×B hybrid embryo samples, indicating preferential maternal expression. (C) The same T/A SNP as in (A) was used to test for allele-specific expression of Blcap_v2a (Fig. 1B). In B×C and C×B newborn brain and whole embryo, Blcap_v2a was expressed from exclusively the paternal allele, in contrast to Blcap_v1a. (D) Allele-specific sequencing of transcripts in the first intron of Blcap_v1a using a T/C SNP between the B6 and cast strains (top gDNA traces and Fig. 1B). Transcripts amplified from B×C and C×B newborn (nb) brain (Br) were predominantly expressed from the paternal allele.