Figure 2.

Effect of CLT on P. falciparum parasitaemia in vitro (A4 clone). Shown are the time- and concentration-dependent effects of continuous incubation with CLT on parasitemia of P. falciparum cultures. All cultures were started with synchronized ring-stage parasites. Parasitemia was measured at the beginning of incubation (0 hr) and every 24 hr thereafter. Because parasite viability was not discernible from morphological criteria alone, morphologically normal and abnormal parasites were included in the measurements. Therefore, “viable parasitemia” may be lower than that shown. Parasitemias in drug-free cultures and cultures with CLT concentrations of 0.5 and 1 μM were diluted with fresh red cells at 72 hr; the values shown at 96 hr were corrected for this dilution.