Figure 4.

Time- and concentration-dependent effects of CLT on P. falciparum stage-specific development in vitro (A4 clone). Ring-stage synchronized cultures were continuously exposed to CLT concentrations of 0.5, 1, 2, 4, and 6 μM. Parasite morphology and stage-specific development were assessed at the beginning of incubation (0 hr) and at 24 and 48 hr. The parasites were classified in four groups: rings (R), late trophozoites (T), schizonts (S), and pyknotic parasites whose stage of development could not be established (P). Parasite differential counts are reported as the percentage of total parasitized cells. The six bars within each group correspond to different CLT concentrations (left to right): 0, 0.5, 1, 2, 4, and 6 μM.