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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Immunity. 2008 Nov 14;29(5):746–757. doi: 10.1016/j.immuni.2008.09.015

Figure 2. Defective central B cell tolerance checkpoint in IRAK-4-, MyD88- and UNC-93B-deficient patients.

Figure 2

(A) Antibodies from new emigrant B cells from controls and patients were tested by ELISA for reactivity against single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), insulin and lipopolysaccharide (LPS). Antibodies are considered polyreactive when they recognize at least 2 and usually all 4 antigens. Dotted lines show ED38-positive control (Wardemann et al., 2003). Horizontal lines show cut-off OD405 for positive reactivity. For each individual, the frequency of polyreactive and non polyreactive clones is summarized in pie charts with the number of antibodies tested indicated in the centers. The frequencies of polyreactive (B) and HEp-2-reactive (C) new emigrant B cells are compared between controls and patients, and statistically significant differences are indicated. Each diamond represents an individual, and the average is shown with a bar.