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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Immunity. 2008 Nov 14;29(5):746–757. doi: 10.1016/j.immuni.2008.09.015

Figure 3. ANA expressing B cell removal requires IRAK-4 and MyD88 but not UNC-93B.

Figure 3

(A) The frequency of anti-nuclear new emigrant B cells in IRAK-4 and MyD88-deficient patients is higher than in controls and UNC-93B-deficient patients. Each diamond represents an individual, and the average is shown with a bar.

(B) The frequencies of chromatin non-reactive and reactive clones in IRAK-4- and MyD88-deficient patients are higher than in controls and UNC-93B-deficient patients. Histograms represent means ± s.e.m. of frequencies of chromatin non-reactive (open bars) or chromatin reactive (black bars) antibodies. Statistically significant differences between patients and controls are indicated.

(C) ANAs from IRAK-4- and MyD88-deficient new emigrant B cells show various patterns of anti-nuclear staining. White arrows indicate dividing cells that demonstrate negative (top row) or positive (bottom row) staining for the condensed chromatin material.

(D) Increased anti-kinetoplast antibodies in new emigrant B cells from IRAK-4- (black bars) and MyD88-deficient (dark grey bars) compared to controls (open bars) and UNC-93B-deficient patients (light grey bars). Histograms represent means ± s.e.m. of proportion of kinetoplast reactive clones. Statistically significant differences between patients and controls are indicated.

(E) IRAK-4- and MyD88-deficient clones express ANAs that can recognize the kinetoplast of C. Luciliae. White and red arrows indicate positively stained kinetoplasts and nucleus of C. Luciliae respectively.

(F) Kinetoplast reactive ANA-expressing B cells display the highest frequency of positively charged residues in their heavy chain CDR3s. New emigrant B cells from IRAK-4 and MyD88-deficient patients were grouped into non-autoreactive, autoreactive-non ANA (HEp-2-reactive and/or polyreactive), kinetoplast non-reactive ANA and kinetoplast reactive ANA clones. Pie charts show the proportion of heavy chain CDR3s with 0, 1, 2 and 3 or more positive charges. Absolute numbers of clones analyzed in each B cell fraction is indicated in the center. The average number of positively charged residues per CDR3 is indicated below each fraction. The numbers of positively charged residues in immunoglobulin heavy chain CDR3s were calculated excluding the arginin or lysin found at position 94 in most germ line VHs.