Fig. 3.

Effects of HIF-1α siRNA on intracellular ROS levels after hypoxia and OGD treatments. A) Representatives of cell images with ROS fluorescence. Images were taken with a 10 objective. Images shown are representative of at least 10 fields of view. B) ROS level. The ROS level was measured with the cell-permeable probe dichlorofluorescin diacetate (DCFH-DA). Cells were incubated with 100 μM DCFH-DA (dissolved in DMSO) for 30 min at 37 °C. N: normal control cells; NC: negative control cells; siRNA: HIF-1α siRNA transfected cells (5 nM). All cells were incubated under normal condition (21% O₂) (Normoxia), hypoxic condition (1% O₂) (Hypoxia), and oxygen and glucose deprivation (OGD) for 3 hrs. The results were normalized by protein content. Data are the mean ± SEM. n=3. *p<0.01 v.s. normal control cells. ^#p<0.01 v.s. negative control cells.