Fig. 5.

The effect of HIF-1α knockdown on the expression of GLUT1, G6PD, PGD and the total cellular NADPH under hypoxic conditions in SH-SY5Y cells. A) Representative western blot of three independent experiments. β-actin serves as protein loading control. B) Average of densitometric analyses normalized to β-actin. C) The total cellular NADPH concentration. N: normal control cells; NC: negative control cells; siRNA: HIF-1α siRNA transfected cells; DFO was used as positive control. All cells were incubated under a hypoxic condition (1% O₂) for 3 hrs. Data are expressed as mean ± SEM (n=3). ^$p<0.05, *p<0.01 v.s. normal control cells; ^& p<0.05, ^#p<0.01 v.s. negative control cells.