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. 2008 Apr 16;28(16):4250–4260. doi: 10.1523/JNEUROSCI.0066-08.2008

Figure 3.

Figure 3.

Effects of α-syn on Notch-1 expression in mES cells. A, Immunoblot analysis of neuronal differentiated cells harvested at stage E8. Proteins in total cell lysates were separated by SDS-PAGE and immunoblotted with anti-β-tubulin, anti-Notch-1 (full-length), anti-NICD, or anti-β-actin. B, Quantification of Notch-1 expression (normalized to β-actin levels). C–E, Double-immunolabeling analysis of differentiated neuronal cells at stage E8 with antibodies against Notch-1 and β-tubulin. Scale bar, 30 μm. F, Levels of Notch-1 immunoreactivity in stage E8 differentiated neuronal cells infected with LV–vector, WT α-syn, or A53T mut α-syn. G, mRNA expression of Notch-1 and Notch-4 as measured by qPCR in stage E8 neuronal differentiated mES cells infected with LV–vector, WT α-syn, or A53T mut α-syn. H, mRNA expression of Jagged and Delta as measured by qPCR in stage E8 neuronal differentiated mES cells infected with LV–vector, WT α-syn, or A53T mut α-syn. I, mRNA expression of Hes-1 and Hes-5 as measured by qPCR in stage E8 neuronal differentiated mES cells infected with LV–vector, WT α-syn, or A53T mut α-syn. J, γ-Secretase activity in stage E8 differentiated mES cells infected with LV–vector, WT α-syn, or A53T mut α-syn. *p < 0.05 compared with vector-infected control cells (one-way ANOVA with post hoc Dunnett's test).