Figure 4.

NICD reversed the decrease in neuronal differentiation and proliferation in α-syn-transduced cells. A, Adenovirus-mediated (MOI of 3) NICD–HA expression in stage E8 neuronal differentiated cells infected with LV–vector, WT α-syn, or A53T mut α-syn. Proteins in total cell lysates were separated by SDS-PAGE and immunoblotted with antibodies against β-tubulin, α-syn, HA, or β-actin. B, NICD expression reversed the decrease in β-tubulin expression in α-syn-transduced cells. C, NICD expression reversed the decrease of cell proliferation in α-syn-transduced cells. Cells were incubated with BrdU (10 μm final concentration) on day E7. After 24 h (E8), the cells were fixed and incubated with anti-BrdU-peroxidase. Peroxidase substrate was added and measured at 405 nm. D, NICD reduced the numbers of TUNEL-positive cells in α-syn-expressing cells. *p < 0.05 compared with vector-infected control cells (one-way ANOVA with post hoc Dunnett's test). OD, Optical density.