Figure 1.

Genome-wide RNAi screen for self-renewal genes in mouse ES cells. (A) Outline of the screen. (Dark wells) Differentiated cells that are GFP-negative; (green wells) self-renewing cells that are GFP-positive. (B) Functional categorization of candidate self-renewal genes identified from the screen. (Left panel) Molecular functions (Panther Classification System). (Right panel) Biological processes (Ingenuity Pathway Analysis). Only selected categories were shown. For complete lists, see Supplemental Table S2. (C) Expression of candidate self-renewal genes during ES cell differentiation. (Right panel) Differentiation after 4 d in RA. (Left panel) Differentiation after 4 d of EB formation. (D) Relative expression level of candidate self-renewal genes in 61 different tissues. Genes were clustered based on their expression levels in embryonic tissues, nervous system, and other tissues.