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. 2009 Apr 1;23(7):824–836. doi: 10.1101/gad.494009

Figure 2.

Figure 2.

GLD-4 is enriched in the germline and associates with P granules. (A) GLD-4 (caret) is germline-enriched. Protein extracts of embryos (E), adult hermaphrodites of given genotypes probed with a rabbit GLD-4(Ct) antibody. (no GL) glp-1(q224ts) animals without germline; (Pos. cont.) Positive control of a LexA∷GLD-4 fusion produced in yeast. (B–F) Extruded germlines (B,C) and embryos (D–F) of wild-type (wt) and gld-4(ef15) adult hermaphrodites stained for chromatin (DAPI), P granules (anti-PGL-1), and GLD-4. (sp) Mature sperm at the proximal end (dashed line). Bar, 50 μm. Insets a–c in B represent a region in the mitotic germline as an optical section generated with an Apotome (Zeiss) microscope. (Inset a) DAPI. (Inset b) PGL-1. (Inset c) GLD-4 staining. (D) Two-cell embryo. (E) Approximately 100-cell embryo. (Arrowheads) Germ cell precursors Z2 and Z3. (F) Epitope-blocked anti-GLD-4(Ct) antibodies (F′′) fail to recognize P granules (F′) in a dividing two-cell wild-type embryo.