Figure 6.

Efficient GLD-1 accumulation is dependent on gld-4 and gld-2 PAPs. (A–L) Extruded distal germlines stained with DAPI (left column) and for GLD-1 (right column). Note that double mutants of the same gene class are shortened and separated by a dash; e.g., fbf-1/2 is a double mutant of fbf-1 and fbf-2. ccr-4i indicates a ccr-4 knockdown by feeding RNAi. (M) Quantification of the immunofluorescence signal from the above germlines. Measured GLD-1 levels from unprocessed images were normalized to wild-type germlines. Data are represented as ±SEM. Genotype labels are as in C–L.