Figure 4.

Autophagy contributes to the transition to the OIS phenotype. (A) SA-β-gal activity and (B) BrdU incorporation in ER:Ras IMR90 expressing the indicated shRNAs at day 4 after 4-OHT treatment. *P < 0.05 relative to vector. (C) The indicated ER:Ras populations of BJ cells were assessed for colony formation in the presence of 4-OHT. (D,E) Autophagy deficiency delays cytokine production as assessed by immunoblotting (D) and qPCR (E) of ER:Ras IMR90 cells. Values are mean ± SEM of at least three experiments (A,B,E).