Figure 6.

The Rho1 PBS is necessary for normal Rho1 function including cytokinesis. (A) Amino acid sequence of Rho1 tail and its mutants as in Figure 5B. (B) The functional importance of the basic residues in the Rho1 tail for growth and cytokinesis. Strains of the indicated genotype were spotted onto plates in serial dilutions. Wild-type RHO1, expressed from the GAL1 promoter, was repressed by growth on medium containing glucose. Images were taken after 3 d growth at the indicated temperature. Cytokinesis failure (more than three connected cells, n = 200) was scored after depletion of wild-type Rho1 for 20 h in glucose-containing medium at 24°C. Cells were sonicated after fixation.