Figure 8.

A model of Rho1 regulation and function in cytokinesis. (Left) CAR assembly in late anaphase. Polo-dependent recruitment of Tus1 promotes CAR assembly by recruiting Rho1 to the division site. After mitotic exit, Chs2 is transported to the bud neck and CAR constriction guides primary septum formation. (Right) After mitotic exit, Polo is degraded and PIP2 plays an important role in concentrating Rho1 activity at the bud neck. See the Discussion for details. (GS) Glucan synthase; (CS) chitin synthase.