Table 1.

Examples of ORFs identified from 2D gel spots (Fig. 1) using SEQUEST searches against the TIGR^a and Los Alamos^b databases for P. gingivalis strain W83

Spots	ORFTIGR	ORFLANL	MWgelc	MWcalcd	Protein Description from LANL annotationsc
1	PG2132	PG1865	70–120	41	major fimbrillin A
2	PG0178	PG0163	88, 42	50d	67 kDa fimbrillin/PGA67/minor fimbrillin A
3	PG2024	PG1768	50	186	arginine-specific cysteine proteinase; gingipain R1
4	PG1844	PG1605	42	188	porphypain polyprotein; lys-X proteinase/hemagglutinin
5	PG1551	PG1357	22	15.5	tonB-dependent receptor HmuY
6	PG1823	PG1592	22	24	probable integral outer membrane protein P20
7	PG2102	PG1838	20	61	LPS-modified surface protein P59
8	PG2124	PG1857	18	36	glyceraldehyde 3-phosphate dehydrogenase
9	PG1858	PG1621	17	19	flavodoxin A

^aTIGR: http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl? database=gpgb.

^bLos Alamos National Lab: http://www.stdgen.lanl.gov/oragen/bacteria/pgin/

^cMolecular weight estimated from spot location on the gel in kDa (see Fig. 1).

^dMolecular weight in kDa from Los Alamos database.