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. Author manuscript; available in PMC: 2009 Apr 7.
Published in final edited form as: Curr Eye Res. 2005 Jul;30(7):527–534. doi: 10.1080/02713680590968150

FIGURE 2.

FIGURE 2

P. aeruginosa induces IL-6 and IL-8 mRNA expression in human corneal epithelial cells. HUCL (panel A) or primary HCE (panel B) cells were grown to ~90% confluence on 6-well plates and were serum-starved overnight. Cells were then infected with P. aeruginosa at a cell-to-bacterium ratio of 1:50 over a 4-hr time course. At the indicated times, cells were lysed, and total RNA was extracted, reverse transcribed, and amplified using IL-6 and IL-8 primers (Table 1) with GAPDH as control. PCR products were separated and stained as described in “Materials and Methods.” Results are representative of three independent experiments.