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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Dec 10;482(1-2):58–65. doi: 10.1016/j.abb.2008.11.028

Figure 5. TFPI-2 interacts with importin-α.

Figure 5

(A), HT-1080 cells overexpressing TFPI-2 and untransfected cells were subjected to co-immunoprecipitation using SK-9 and importin-α antibodies as described in Methods. The complexes were immunoblotted separately using anti-TFPI-2 and anti-importin-α antibodies. Lane 1, HT-1080 untransfected cell lysate co-immunoprecipitated with SK-9; lane 2, HT-1080 cells overexpressing TFPI-2 co-immunoprecipitated with SK-9; lane 3, HT-1080 untransfected cell lysate co-immunoprecipitated with anti-importin-α; lane 4, HT-1080 cells overexpressing TFPI-2 co-immunoprecipitated with anti-importin-α. For immunoblot analysis, the intensity of blot bands were assessed by densitometric semi-quantitation and depicted by a bar diagram (lower panel). (B), HT-1080 cells were treated with 1 μM TFPI-2 and processed for co-immunoprecipitation. The complexes were similarly immunoblotted using anti-TFPI-2 and anti-importin-α antibodies. Lane 1, lysate from vehicle treated HT-1080 cells co-immunoprecipitated with SK-9; lane 2, lysate from TFPI-2 treated HT-1080 cells co-immunoprecipitated with SK-9; lane 3, lysate from vehicle treated HT-1080 cells co-immunoprecipitated with anti-importin-α; lane 4, lysate from TFPI-2 treated HT-1080 cells co-immunoprecipitated with anti-importin-α. For immunoblot analysis, the intensity of blot bands were assessed by densitometric semi-quantitation and depicted by a bar diagram (lower panel).