Figure 3.—

Combining known suppressors of rad57 fully suppresses the sensitivity of rad57 to DSB-inducing genotoxic agents. (A) LSY1519-1D (RAD), LSY1896-1A (rad57∷LEU2 srs2∷HIS3 rad51-I345T), LSY1422-6B (rad57∷LEU2 srs2∷HIS3), LSY1895 (rad57∷LEU2 rad51-I345T), LSY 1894-3B (rad57∷LEU2), LSY1827-1D (srs2∷HIS3 rad51-I345T), and LSY1867 (srs2∷HIS3) were transformed with either pRS414-MATα or pRS414-MATa to express both mating-type alleles or pRS414 empty vector as a control. Ten-fold serial dilutions of log-phase SC–TRP cultures grown at 30° were spotted onto SC–TRP plates and left unirradiated or exposed to 800 Gy of γ-irradiation. Dilutions were also spotted onto SC–TRP plates containing 1 μg/ml camptothecin buffered with 0.25% DMSO. Survival was assessed following 3 days of growth at 30°. (B) The same experiment as in A done at 23°.