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. 2009 Apr;181(4):1195–1206. doi: 10.1534/genetics.109.100842

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Copyright © 2009 by the Genetics Society of America

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Figure 5.— — The spontaneous recombination repair defect of rad57 is weakly suppressed by combining srs2 and mating-type heterozygosity. (A) The direct-repeat recombination substrate contains 3.6-kb repeats with different ade2 alleles integrated at the endogenous locus on chromosome XV separated by plasmid sequences and the URA3 gene. Unequal sister-chromatid or intrachromatid gene conversion between the two ade2 repeats can generate Ade⁺Ura⁺ recombinants that retain the duplication. Either allele could be converted; only one type of conversion is shown here. (B) Spontaneous sister-chromatid recombination rates at 30°. Strains used were LSY1892 (RAD), LSY1894-3B (rad57∷LEU2), LSY1898 (rad57:LEU2 sir4∷kanMX), LSY1422-6B (rad57∷LEU2 srs2∷HIS3), LSY1421-2A (srs2∷HIS3), and LSY1900 (rad57∷LEU2 srs2∷HIS3 sir4∷KanMX).