Figure 6.—

Mating-type heterozygosity and srs2 have different effects on rad51-I345T foci. (A) LSY1957-1 (MATa YFP-rad51-I345T) was transformed with pRS414 empty vector or pRS414-MATα to express both mating types, and LSY2086-2B (YFP-rad51-I345T srs2∷HIS3) was transformed with pRS414 empty vector. Log-phase cultures of the strains were exposed to 200 Gy of γ-irradiation, followed by microscopy to monitor focus formation. (B) The brightness of each focus was quantified and plotted; at least 50 foci were analyzed for each strain. A solid bar represents the mean focus brightness for each strain.