Figure 7.—

MAT heterozygosity does not suppress the IR sensitivity of rad57 by downregulating NHEJ. Strains used were W1588-4A (RAD), YHK598-8B (rad57∷LEU2), LSY2029 (rad57∷LEU2 dnl4∷KanMX), LSY1682-1B (rad55∷LEU2 dnl4∷URA3), LSY1786 (rad55∷LEU2 dnl4∷KanMX), and BY4742 rad55∷KanMX. To express both mating types, YHK598-8B (MATα rad57∷LEU2) was transformed with pRS414-MATa while the rest of the strains were transformed with the pRS414 empty vector. Ten-fold serial dilutions of log-phase cultures were spotted onto either SC–TRP-only plates or onto SC–TRP plates containing 1 μg/ml camptothecin buffered with 0.25% DMSO. SC–TRP-only plates either were left unirradiated or were exposed to 400 Gy of γ-irradiation.